######## snakemake preamble start (automatically inserted, do not edit) ########
import sys;sys.path.extend(['/home/aloes/miniforge3/envs/seqneut-pipeline/lib/python3.12/site-packages', '/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/seqneut-pipeline', '/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts', '/home/aloes/miniforge3/envs/seqneut-pipeline/bin', '/home/aloes/miniforge3/envs/seqneut-pipeline/lib/python3.12', '/home/aloes/miniforge3/envs/seqneut-pipeline/lib/python3.12/lib-dynload', '/home/aloes/miniforge3/envs/seqneut-pipeline/lib/python3.12/site-packages', '/home/aloes/.cache/snakemake/snakemake/source-cache/runtime-cache/tmpt_2xtaev/file/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/seqneut-pipeline/notebooks', '/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/seqneut-pipeline/notebooks']);import pickle;from snakemake import script;script.snakemake = 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script;from snakemake.logging import logger;from snakemake.script import snakemake; logger.printshellcmds = False;import os; os.chdir(r'/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts');
######## snakemake preamble end #########

Process plate counts to get fraction infectivities and fit curves

This notebook is designed to be run using snakemake, and analyzes a plate of sequencing-based neutralization assays.

The plots generated by this notebook are interactive, so you can mouseover points for details, use the mouse-scroll to zoom and pan, and use interactive dropdowns at the bottom of the plots.

Setup

Import Python modules:

import pickle
import sys

import altair as alt

import matplotlib.pyplot as plt

import neutcurve

import numpy

import pandas as pd

import ruamel.yaml as yaml

_ = alt.data_transformers.disable_max_rows()

Get the variables passed by snakemake:

count_csvs = snakemake.input.count_csvs
fate_csvs = snakemake.input.fate_csvs
viral_library_csv = snakemake.input.viral_library_csv
neut_standard_set_csv = snakemake.input.neut_standard_set_csv
qc_drops_yaml = snakemake.output.qc_drops
frac_infectivity_csv = snakemake.output.frac_infectivity_csv
fits_csv = snakemake.output.fits_csv
fits_pickle = snakemake.output.fits_pickle
samples = snakemake.params.samples
plate = snakemake.wildcards.plate
plate_params = snakemake.params.plate_params

# get thresholds turning lists into tuples as needed
manual_drops = {
    filter_type: [tuple(w) if isinstance(w, list) else w for w in filter_drops]
    for (filter_type, filter_drops) in plate_params["manual_drops"].items()
}
group = plate_params["group"]
qc_thresholds = plate_params["qc_thresholds"]
curvefit_params = plate_params["curvefit_params"]
curvefit_qc = plate_params["curvefit_qc"]
curvefit_qc["barcode_serum_replicates_ignore_curvefit_qc"] = [
    tuple(w) for w in curvefit_qc["barcode_serum_replicates_ignore_curvefit_qc"]
]

print(f"Processing {plate=}")

samples_df = pd.DataFrame(plate_params["samples"])
print(f"\nPlate has {len(samples)} samples (wells)")
assert all(
    (len(samples_df) == samples_df[c].nunique())
    for c in ["well", "sample", "sample_noplate"]
)
assert len(samples_df) == len(
    samples_df.groupby(["serum_replicate", "dilution_factor"])
)
assert len(samples) == len(count_csvs) == len(fate_csvs) == len(samples_df)

for d, key, title in [
    (manual_drops, "manual_drops", "Data manually specified to drop:"),
    (qc_thresholds, "qc_thresholds", "QC thresholds applied to data:"),
    (curvefit_params, "curvefit_params", "Curve-fitting parameters:"),
    (curvefit_qc, "curvefit_qc", "Curve-fitting QC:"),
]:
    print(f"\n{title}")
    yaml.YAML(typ="rt").dump({key: d}, stream=sys.stdout)

Processing plate='plate6'

Plate has 96 samples (wells)

Data manually specified to drop:
manual_drops:
  serum_replicates:
  - PENN23_y1964_s038_d0
  - PENN23_y1964_s038_d28
  wells:
  - A12
  - B12
  - C12
  - D12
  - E12
  - F12
  - G12
  - H12

QC thresholds applied to data:
qc_thresholds:
  avg_barcode_counts_per_well: 500
  min_neut_standard_frac_per_well: 0.005
  no_serum_per_viral_barcode_filters:
    min_frac: 4e-05
    max_fold_change: 4
    max_wells: 2
  per_neut_standard_barcode_filters:
    min_frac: 0.0002
    max_fold_change: 4
    max_wells: 2
  min_neut_standard_count_per_well: 500
  min_no_serum_count_per_viral_barcode_well: 50
  max_frac_infectivity_per_viral_barcode_well: 4
  min_dilutions_per_barcode_serum_replicate: 5

Curve-fitting parameters:
curvefit_params:
  frac_infectivity_ceiling: 1
  fixtop:
  - 0.6
  - 1
  fixbottom: 0
  fixslope:
  - 0.8
  - 10

Curve-fitting QC:
curvefit_qc:
  max_frac_infectivity_at_least: 0
  goodness_of_fit:
    min_R2: 0.7
    max_RMSD: 0.2
  serum_replicates_ignore_curvefit_qc: []
  barcode_serum_replicates_ignore_curvefit_qc: []

Set up dictionary to keep track of wells, barcodes, well-barcodes, and serum-replicates that are dropped:

qc_drops = {
    "wells": {},
    "barcodes": {},
    "barcode_wells": {},
    "barcode_serum_replicates": {},
    "serum_replicates": {},
}

assert set(manual_drops).issubset(
    qc_drops
), f"{manual_drops.keys()=}, {qc_drops.keys()}"

Statistics on barcode-parsing for each sample

Make interactive chart of the "fates" of the sequencing reads parsed for each sample on the plate.

If most sequencing reads are not "valid barcodes", this could potentially indicate some problem in the sequencing or barcode set you are parsing.

Potential fates are:

• valid barcode: barcode that matches a known virus or neutralization standard, we hope most reads are this.
• invalid barcode: a barcode with proper flanking sequences, but does not match a known virus or neutralization standard. If you have a lot of reads of this type, it is probably a good idea to look at the invalid barcode CSVs (in the ./results/barcode_invalid/ subdirectory created by the pipeline) to see what these invalid barcodes are.
• unparseable barcode: could not parse a barcode from this read as there was not a sequence of the correct length with the appropriate flanking sequence.
• invalid outer flank: if using an outer upstream or downstream region (upstream2 or downstream2 for the illuminabarcodeparser), reads that are otherwise valid except for this outer flank. Typically you would be using upstream2 if you have a plate index embedded in your primer, and reads with this classification correspond to a different index than the one for this plate.
• low quality barcode: low-quality or N nucleotides in barcode, could indicate problem with sequencing.
• failed chastity filter: reads that failed the Illumina chastity filter, if these are reported in the FASTQ (they may not be).

Also, if the number of reads per sample is very uneven, that could indicate that you did not do a good job of balancing the different samples in the Illumina sequencing.

fates = (
    pd.concat([pd.read_csv(f).assign(sample=s) for f, s in zip(fate_csvs, samples)])
    .merge(samples_df, validate="many_to_one", on="sample")
    .assign(
        fate_counts=lambda x: x.groupby("fate")["count"].transform("sum"),
        sample_well=lambda x: x["sample_noplate"] + " (" + x["well"] + ")",
    )
    .query("fate_counts > 0")[  # only keep fates with at least one count
        ["fate", "count", "well", "serum_replicate", "sample_well", "dilution_factor"]
    ]
)

assert len(fates) == len(fates.drop_duplicates())

serum_replicates = sorted(fates["serum_replicate"].unique())
sample_wells = list(
    fates.sort_values(["serum_replicate", "dilution_factor"])["sample_well"]
)


serum_selection = alt.selection_point(
    fields=["serum_replicate"],
    bind=alt.binding_select(
        options=[None] + serum_replicates,
        labels=["all"] + serum_replicates,
        name="serum",
    ),
)

fates_chart = (
    alt.Chart(fates)
    .add_params(serum_selection)
    .transform_filter(serum_selection)
    .encode(
        alt.X("count", scale=alt.Scale(nice=False, padding=3)),
        alt.Y(
            "sample_well",
            title=None,
            sort=sample_wells,
        ),
        alt.Color("fate", sort=sorted(fates["fate"].unique(), reverse=True)),
        alt.Order("fate", sort="descending"),
        tooltip=fates.columns.tolist(),
    )
    .mark_bar(height={"band": 0.85})
    .properties(
        height=alt.Step(10),
        width=200,
        title=f"Barcode parsing for {plate}",
    )
    .configure_axis(grid=False)
)

fates_chart

Read barcode counts and apply manually specified drops

Read the counts per barcode:

# get barcode counts
counts = (
    pd.concat([pd.read_csv(c).assign(sample=s) for c, s in zip(count_csvs, samples)])
    .merge(samples_df, validate="many_to_one", on="sample")
    .drop(columns=["replicate", "plate", "fastq"])
    .assign(sample_well=lambda x: x["sample_noplate"] + " (" + x["well"] + ")")
)

# classify barcodes as viral or neut standard
barcode_class = pd.concat(
    [
        pd.read_csv(viral_library_csv)[["barcode", "strain"]].assign(
            neut_standard=False,
        ),
        pd.read_csv(neut_standard_set_csv)[["barcode"]].assign(
            neut_standard=True,
            strain=pd.NA,
        ),
    ],
    ignore_index=True,
)

# merge counts and classification of barcodes
assert set(counts["barcode"]) == set(barcode_class["barcode"])
counts = counts.merge(barcode_class, on="barcode", validate="many_to_one")
assert set(sample_wells) == set(counts["sample_well"])
assert set(serum_replicates) == set(counts["serum_replicate"])

Apply any manually specified data drops:

for filter_type, filter_drops in manual_drops.items():
    print(f"\nDropping {len(filter_drops)} {filter_type} specified in manual_drops")
    assert filter_type in qc_drops
    qc_drops[filter_type].update(
        {w: "manual_drop" for w in filter_drops if not isinstance(w, list)}
    )
    if filter_type == "barcode_wells":
        counts = counts[
            ~counts.assign(
                barcode_well=lambda x: x.apply(
                    lambda r: (r["barcode"], r["well"]), axis=1
                )
            )["barcode_well"].isin(qc_drops[filter_type])
        ]
    elif filter_type == "barcode_serum_replicates":
        counts = counts[
            ~counts.assign(
                barcode_serum_replicate=lambda x: x.apply(
                    lambda r: (r["barcode"], r["serum_replicate"]), axis=1
                )
            )["barcode_serum_replicate"].isin(qc_drops[filter_type])
        ]
    elif filter_type == "wells":
        counts = counts[~counts["well"].isin(qc_drops[filter_type])]
    elif filter_type == "barcodes":
        counts = counts[~counts["barcode"].isin(qc_drops[filter_type])]
    elif filter_type == "serum_replicates":
        counts = counts[~counts["serum_replicate"].isin(qc_drops[filter_type])]
    elif filter_type == "barcode_serum_replicates":
        counts = counts[~counts["barcode_serum_replicate"].isin(qc_drops[filter_type])]
    else:
        assert filter_type in set(counts.columns)
        counts = counts[~counts[filter_type].isin(qc_drops[filter_type])]

Dropping 2 serum_replicates specified in manual_drops

Dropping 8 wells specified in manual_drops

Average counts per barcode in each well

Plot average counts per barcode. If a sample has inadequate barcode counts, it may not have good enough statistics for accurate analysis, and a QC-threshold is applied:

avg_barcode_counts = (
    counts.groupby(
        ["well", "serum_replicate", "sample_well"],
        dropna=False,
        as_index=False,
    )
    .aggregate(avg_count=pd.NamedAgg("count", "mean"))
    .assign(
        fails_qc=lambda x: (
            x["avg_count"] < qc_thresholds["avg_barcode_counts_per_well"]
        ),
    )
)

avg_barcode_counts_chart = (
    alt.Chart(avg_barcode_counts)
    .add_params(serum_selection)
    .transform_filter(serum_selection)
    .encode(
        alt.X(
            "avg_count",
            title="average barcode counts per well",
            scale=alt.Scale(nice=False, padding=3),
        ),
        alt.Y("sample_well", sort=sample_wells),
        alt.Color(
            "fails_qc",
            title=f"fails {qc_thresholds['avg_barcode_counts_per_well']=}",
            legend=alt.Legend(titleLimit=500),
        ),
        tooltip=[
            alt.Tooltip(c, format=".3g") if avg_barcode_counts[c].dtype == float else c
            for c in avg_barcode_counts.columns
        ],
    )
    .mark_bar(height={"band": 0.85})
    .properties(
        height=alt.Step(10),
        width=250,
        title=f"Average barcode counts per well for {plate}",
    )
    .configure_axis(grid=False)
)

display(avg_barcode_counts_chart)

# drop wells failing QC
avg_barcode_counts_per_well_drops = list(avg_barcode_counts.query("fails_qc")["well"])
print(
    f"\nDropping {len(avg_barcode_counts_per_well_drops)} wells for failing "
    f"{qc_thresholds['avg_barcode_counts_per_well']=}: "
    + str(avg_barcode_counts_per_well_drops)
)
qc_drops["wells"].update(
    {w: "avg_barcode_counts_per_well" for w in avg_barcode_counts_per_well_drops}
)
counts = counts[~counts["well"].isin(qc_drops["wells"])]

Dropping 0 wells for failing qc_thresholds['avg_barcode_counts_per_well']=500: []

Fraction of counts from neutralization standard

Determine the fraction of counts from the neutralization standard in each sample, and make sure this fraction passess the QC threshold.

neut_standard_fracs = (
    counts.assign(
        neut_standard_count=lambda x: x["count"] * x["neut_standard"].astype(int)
    )
    .groupby(
        ["well", "serum_replicate", "sample_well"],
        dropna=False,
        as_index=False,
    )
    .aggregate(
        total_count=pd.NamedAgg("count", "sum"),
        neut_standard_count=pd.NamedAgg("neut_standard_count", "sum"),
    )
    .assign(
        neut_standard_frac=lambda x: x["neut_standard_count"] / x["total_count"],
        fails_qc=lambda x: (
            x["neut_standard_frac"] < qc_thresholds["min_neut_standard_frac_per_well"]
        ),
    )
)

neut_standard_fracs_chart = (
    alt.Chart(neut_standard_fracs)
    .add_params(serum_selection)
    .transform_filter(serum_selection)
    .encode(
        alt.X(
            "neut_standard_frac",
            title="frac counts from neutralization standard per well",
            scale=alt.Scale(nice=False, padding=3),
        ),
        alt.Y("sample_well", sort=sample_wells),
        alt.Color(
            "fails_qc",
            title=f"fails {qc_thresholds['min_neut_standard_frac_per_well']=}",
            legend=alt.Legend(titleLimit=500),
        ),
        tooltip=[
            alt.Tooltip(c, format=".3g") if neut_standard_fracs[c].dtype == float else c
            for c in neut_standard_fracs.columns
        ],
    )
    .mark_bar(height={"band": 0.85})
    .properties(
        height=alt.Step(10),
        width=250,
        title=f"Neutralization-standard fracs per well for {plate}",
    )
    .configure_axis(grid=False)
    .configure_legend(titleLimit=1000)
)

display(neut_standard_fracs_chart)

# drop wells failing QC
min_neut_standard_frac_per_well_drops = list(
    neut_standard_fracs.query("fails_qc")["well"]
)
print(
    f"\nDropping {len(min_neut_standard_frac_per_well_drops)} wells for failing "
    f"{qc_thresholds['min_neut_standard_frac_per_well']=}: "
    + str(min_neut_standard_frac_per_well_drops)
)
qc_drops["wells"].update(
    {
        w: "min_neut_standard_frac_per_well"
        for w in min_neut_standard_frac_per_well_drops
    }
)
counts = counts[~counts["well"].isin(qc_drops["wells"])]

Dropping 0 wells for failing qc_thresholds['min_neut_standard_frac_per_well']=0.005: []

Consistency and minimum fractions for barcodes

We examine the fraction of counts attributable to each barcode. We do this splitting the data two ways:

1. Looking at all viral (but not neut-standard) barcodes only for the no-serum samples (wells).

2. Looking at just the neut-standard barcodes for all samples (wells).

The reasons is that if the experiment is set up perfectly, these fractions should be the same across all samples for each barcode. (We do not expect viral barcodes to have consistent fractions across no-serum samples as they will be neutralized differently depending on strain).

We plot these fractions in interactive plots (you can mouseover points and zoom) so you can identify barcodes that fail the expected consistency QC thresholds.

We also make sure the barcodes meet specified QC minimum thresholds for all samples, and flag any that do not.

barcode_selection = alt.selection_point(fields=["barcode"], on="mouseover", empty=False)

# look at all samples for neut standard barcodes, or no-serum samples for all barcodes
for is_neut_standard, df in counts.groupby("neut_standard"):
    if is_neut_standard:
        print(
            f"\n\n{'=' * 89}\nAnalyzing neut-standard barcodes from all samples (wells)"
        )
        qc_name = "per_neut_standard_barcode_filters"
    else:
        print(f"\n\n{'=' * 89}\nAnalyzing all barcodes from no-serum samples (wells)")
        qc_name = "no_serum_per_viral_barcode_filters"
        df = df.query("serum == 'none'")

    df = df.assign(
        sample_counts=lambda x: x.groupby("sample")["count"].transform("sum"),
        count_frac=lambda x: x["count"] / x["sample_counts"],
        median_count_frac=lambda x: x.groupby("barcode")["count_frac"].transform(
            "median"
        ),
        fold_change_from_median=lambda x: numpy.where(
            x["count_frac"] > x["median_count_frac"],
            x["count_frac"] / x["median_count_frac"],
            x["median_count_frac"] / x["count_frac"],
        ),
    )[
        [
            "barcode",
            "count",
            "well",
            "sample_well",
            "count_frac",
            "median_count_frac",
            "fold_change_from_median",
        ]
        + ([] if is_neut_standard else ["strain"])
    ]

    # barcode fails QC if fails in sufficient wells
    qc = qc_thresholds[qc_name]
    print(f"Apply QC {qc_name}: {qc}\n")
    fails_qc = (
        df.assign(
            fails_qc=lambda x: ~(
                (x["count_frac"] >= qc["min_frac"])
                & (x["fold_change_from_median"] <= qc["max_fold_change"])
            ),
        )
        .groupby("barcode", as_index=False)
        .aggregate(n_wells_fail_qc=pd.NamedAgg("fails_qc", "sum"))
        .assign(fails_qc=lambda x: x["n_wells_fail_qc"] >= qc["max_wells"])[
            ["barcode", "fails_qc"]
        ]
    )
    df = df.merge(fails_qc, on="barcode", validate="many_to_one")

    # make chart
    evenness_chart = (
        alt.Chart(df)
        .add_params(barcode_selection)
        .encode(
            alt.X(
                "count_frac",
                title=(
                    "barcode's fraction of neut standard counts"
                    if is_neut_standard
                    else "barcode's fraction of non-neut standard counts"
                ),
                scale=alt.Scale(nice=False, padding=5),
            ),
            alt.Y("sample_well", sort=sample_wells),
            alt.Fill(
                "fails_qc",
                title=f"fails {qc_name}",
                legend=alt.Legend(titleLimit=500),
            ),
            strokeWidth=alt.condition(barcode_selection, alt.value(2), alt.value(0)),
            size=alt.condition(barcode_selection, alt.value(60), alt.value(35)),
            tooltip=[
                alt.Tooltip(c, format=".2g") if df[c].dtype == float else c
                for c in df.columns
            ],
        )
        .mark_circle(fillOpacity=0.45, stroke="black", strokeOpacity=1)
        .properties(
            height=alt.Step(10),
            width=300,
            title=alt.TitleParams(
                (
                    f"{plate} all samples, neut-standard barcodes"
                    if is_neut_standard
                    else f"{plate} no-serum samples, all barcodes"
                ),
                subtitle="x-axis is zoomable (use mouse scroll/pan)",
            ),
        )
        .configure_axis(grid=False)
        .configure_legend(titleLimit=1000)
        .interactive()
    )

    display(evenness_chart)

    # drop barcodes failing QC
    barcode_drops = list(fails_qc.query("fails_qc")["barcode"])
    print(
        f"\nDropping {len(barcode_drops)} barcodes for failing {qc=}: {barcode_drops}"
    )
    qc_drops["barcodes"].update(
        {bc: "min_neut_standard_frac_per_well" for bc in barcode_drops}
    )
    counts = counts[~counts["barcode"].isin(qc_drops["barcodes"])]

=========================================================================================
Analyzing all barcodes from no-serum samples (wells)
Apply QC no_serum_per_viral_barcode_filters: {'min_frac': 4e-05, 'max_fold_change': 4, 'max_wells': 2}

Dropping 4 barcodes for failing qc={'min_frac': 4e-05, 'max_fold_change': 4, 'max_wells': 2}: ['CCAGGAACAATATATC', 'CCCACCCCTGCCTCCC', 'GAGCAGTATCTGGTTA', 'TTGAAAGCCACAACAC']


=========================================================================================
Analyzing neut-standard barcodes from all samples (wells)
Apply QC per_neut_standard_barcode_filters: {'min_frac': 0.0002, 'max_fold_change': 4, 'max_wells': 2}

Dropping 0 barcodes for failing qc={'min_frac': 0.0002, 'max_fold_change': 4, 'max_wells': 2}: []

Compute fraction infectivity

The fraction infectivity for viral barcode $v_b$ in sample $s$ is computed as: $$ F_{v_b,s} = \frac{c_{v_b,s} / \left(\sum_{n_b} c_{n_b,s}\right)}{{\rm median}_{s_0}\left[ c_{v_b,s_0} / \left(\sum_{n_b} c_{n_b,s_0}\right)\right]} $$ where

• $c_{v_b,s}$ is the counts of viral barcode $v_b$ in sample $s$.
• $\sum_{n_b} c_{n_b,s}$ is the sum of the counts for all neutralization standard barcodes $n_b$ for sample $s$.
• $c_{v_b,s_0}$ is the counts of viral barcode $v_b$ in no-serum sample $s_0$.
• $\sum_{n_b} c_{n_b,s_0}$ is the sum of the counts for all neutralization standard barcodes $n_b$ for no-serum sample $s_0$.
• ${\rm median}_{s_0}\left[ c_{v_b,s_0} / \left(\sum_{n_b} c_{n_b,s_0}\right)\right]$ is the median taken across all no-serum samples of the counts of viral barcode $v_b$ versus the total counts for all neutralization standard barcodes.

First, compute the total neutralization-standard counts for each sample (well). Plot these, and drop any wells that do not meet the QC threshold.

neut_standard_counts = (
    counts.query("neut_standard")
    .groupby(
        ["well", "serum_replicate", "sample_well", "dilution_factor"],
        dropna=False,
        as_index=False,
    )
    .aggregate(neut_standard_count=pd.NamedAgg("count", "sum"))
    .assign(
        fails_qc=lambda x: (
            x["neut_standard_count"] < qc_thresholds["min_neut_standard_count_per_well"]
        ),
    )
)

neut_standard_counts_chart = (
    alt.Chart(neut_standard_counts)
    .add_params(serum_selection)
    .transform_filter(serum_selection)
    .encode(
        alt.X(
            "neut_standard_count",
            title="counts from neutralization standard",
            scale=alt.Scale(nice=False, padding=3),
        ),
        alt.Y("sample_well", sort=sample_wells),
        alt.Color(
            "fails_qc",
            title=f"fails {qc_thresholds['min_neut_standard_count_per_well']=}",
            legend=alt.Legend(titleLimit=500),
        ),
        tooltip=[
            (
                alt.Tooltip(c, format=".3g")
                if neut_standard_counts[c].dtype == float
                else c
            )
            for c in neut_standard_counts.columns
        ],
    )
    .mark_bar(height={"band": 0.85})
    .properties(
        height=alt.Step(10),
        width=250,
        title=f"Neutralization-standard counts for {plate}",
    )
    .configure_axis(grid=False)
    .configure_legend(titleLimit=1000)
)

display(neut_standard_counts_chart)

# drop wells failing QC
min_neut_standard_count_per_well_drops = list(
    neut_standard_counts.query("fails_qc")["well"]
)
print(
    f"\nDropping {len(min_neut_standard_count_per_well_drops)} wells for failing "
    f"{qc_thresholds['min_neut_standard_count_per_well']=}: "
    + str(min_neut_standard_count_per_well_drops)
)
qc_drops["wells"].update(
    {
        w: "min_neut_standard_count_per_well"
        for w in min_neut_standard_count_per_well_drops
    }
)
neut_standard_counts = neut_standard_counts[
    ~neut_standard_counts["well"].isin(qc_drops["wells"])
]
counts = counts[~counts["well"].isin(qc_drops["wells"])]

Dropping 0 wells for failing qc_thresholds['min_neut_standard_count_per_well']=500: []

Compute and plot the no-serum sample viral barcode counts and check if they pass the QC filters.

no_serum_counts = (
    counts.query("serum == 'none'")
    .query("not neut_standard")
    .merge(neut_standard_counts, validate="many_to_one")[
        ["barcode", "strain", "well", "sample_well", "count", "neut_standard_count"]
    ]
    .assign(
        fails_qc=lambda x: (
            x["count"] <= qc_thresholds["min_no_serum_count_per_viral_barcode_well"]
        ),
    )
)

strains = sorted(no_serum_counts["strain"].unique())
strain_selection_dropdown = alt.selection_point(
    fields=["strain"],
    bind=alt.binding_select(
        options=[None] + strains,
        labels=["all"] + strains,
        name="virus strain",
    ),
)

# make chart
no_serum_counts_chart = (
    alt.Chart(no_serum_counts)
    .add_params(barcode_selection, strain_selection_dropdown)
    .transform_filter(strain_selection_dropdown)
    .encode(
        alt.X(
            "count", title="viral barcode count", scale=alt.Scale(nice=False, padding=5)
        ),
        alt.Y("sample_well", sort=sample_wells),
        alt.Fill(
            "fails_qc",
            title=f"fails {qc_thresholds['min_no_serum_count_per_viral_barcode_well']=}",
            legend=alt.Legend(titleLimit=500),
        ),
        strokeWidth=alt.condition(barcode_selection, alt.value(2), alt.value(0)),
        size=alt.condition(barcode_selection, alt.value(60), alt.value(35)),
        tooltip=no_serum_counts.columns.tolist(),
    )
    .mark_circle(fillOpacity=0.6, stroke="black", strokeOpacity=1)
    .properties(
        height=alt.Step(10),
        width=400,
        title=f"{plate} viral barcode counts in no-serum samples",
    )
    .configure_axis(grid=False)
    .configure_legend(titleLimit=1000)
    .interactive()
)

display(no_serum_counts_chart)

# drop barcode / wells failing QC
min_no_serum_count_per_viral_barcode_well_drops = list(
    no_serum_counts.query("fails_qc")[["barcode", "well"]].itertuples(
        index=False, name=None
    )
)
print(
    f"\nDropping {len(min_no_serum_count_per_viral_barcode_well_drops)} barcode-wells for failing "
    f"{qc_thresholds['min_no_serum_count_per_viral_barcode_well']=}: "
    + str(min_no_serum_count_per_viral_barcode_well_drops)
)
qc_drops["barcode_wells"].update(
    {
        w: "min_no_serum_count_per_viral_barcode_well"
        for w in min_no_serum_count_per_viral_barcode_well_drops
    }
)
no_serum_counts = no_serum_counts[
    ~no_serum_counts.assign(
        barcode_well=lambda x: x.apply(lambda r: (r["barcode"], r["well"]), axis=1)
    )["barcode_well"].isin(qc_drops["barcode_wells"])
]
counts = counts[
    ~counts.assign(
        barcode_well=lambda x: x.apply(lambda r: (r["barcode"], r["well"]), axis=1)
    )["barcode_well"].isin(qc_drops["barcode_wells"])
]

Dropping 1 barcode-wells for failing qc_thresholds['min_no_serum_count_per_viral_barcode_well']=50: [('AAAATCATTCGACTCT', 'G1')]

Compute and plot the median ratio of viral barcode count to neut standard counts across no-serum samples. If library composition is equal, all of these values should be similar:

median_no_serum_ratio = (
    no_serum_counts.assign(ratio=lambda x: x["count"] / x["neut_standard_count"])
    .groupby(["barcode", "strain"], as_index=False)
    .aggregate(median_no_serum_ratio=pd.NamedAgg("ratio", "median"))
)

strain_selection = alt.selection_point(fields=["strain"], on="mouseover", empty=False)

median_no_serum_ratio_chart = (
    alt.Chart(median_no_serum_ratio)
    .add_params(strain_selection)
    .encode(
        alt.X(
            "median_no_serum_ratio",
            title="median ratio of counts",
            scale=alt.Scale(nice=False, padding=5),
        ),
        alt.Y(
            "barcode",
            sort=alt.SortField("median_no_serum_ratio", order="descending"),
            axis=alt.Axis(labelFontSize=5),
        ),
        color=alt.condition(strain_selection, alt.value("orange"), alt.value("gray")),
        tooltip=[
            (
                alt.Tooltip(c, format=".3g")
                if median_no_serum_ratio[c].dtype == float
                else c
            )
            for c in median_no_serum_ratio.columns
        ],
    )
    .mark_bar(height={"band": 0.85})
    .properties(
        height=alt.Step(5),
        width=250,
        title=f"{plate} no-serum median ratio viral barcode to neut-standard barcode",
    )
    .configure_axis(grid=False)
    .configure_legend(titleLimit=1000)
)

display(median_no_serum_ratio_chart)

Compute the actual fraction infectivities. We compute both the raw fraction infectivities and the ones with the ceiling applied:

frac_infectivity = (
    counts.query("not neut_standard")
    .query("serum != 'none'")
    .merge(median_no_serum_ratio, validate="many_to_one")
    .merge(neut_standard_counts, validate="many_to_one")
    .assign(
        frac_infectivity_raw=lambda x: (
            (x["count"] / x["neut_standard_count"]) / x["median_no_serum_ratio"]
        ),
        frac_infectivity_ceiling=lambda x: x["frac_infectivity_raw"].clip(
            upper=curvefit_params["frac_infectivity_ceiling"]
        ),
        concentration=lambda x: 1 / x["dilution_factor"],
        plate_barcode=lambda x: x["plate_replicate"] + "-" + x["barcode"],
    )[
        [
            "barcode",
            "plate_barcode",
            "well",
            "strain",
            "serum",
            "serum_replicate",
            "dilution_factor",
            "concentration",
            "frac_infectivity_raw",
            "frac_infectivity_ceiling",
        ]
    ]
)

assert len(
    frac_infectivity.groupby(["serum", "plate_barcode", "dilution_factor"])
) == len(frac_infectivity)
assert frac_infectivity["dilution_factor"].notnull().all()
assert frac_infectivity["frac_infectivity_raw"].notnull().all()
assert frac_infectivity["frac_infectivity_ceiling"].notnull().all()

Plot the fraction infectivities, both the raw values and with the ceiling applied:

frac_infectivity_chart_df = (
    frac_infectivity.assign(
        fails_qc=lambda x: (
            x["frac_infectivity_raw"]
            > qc_thresholds["max_frac_infectivity_per_viral_barcode_well"]
        ),
    )
    .melt(
        id_vars=[
            "barcode",
            "strain",
            "well",
            "serum_replicate",
            "dilution_factor",
            "fails_qc",
        ],
        value_vars=["frac_infectivity_raw", "frac_infectivity_ceiling"],
        var_name="ceiling_applied",
        value_name="frac_infectivity",
    )
    .assign(
        ceiling_applied=lambda x: x["ceiling_applied"].map(
            {
                "frac_infectivity_raw": "raw fraction infectivity",
                "frac_infectivity_ceiling": f"fraction infectivity with ceiling at {curvefit_params['frac_infectivity_ceiling']}",
            }
        )
    )
)

frac_infectivity_chart = (
    alt.Chart(frac_infectivity_chart_df)
    .add_params(strain_selection_dropdown, barcode_selection)
    .transform_filter(strain_selection_dropdown)
    .encode(
        alt.X(
            "dilution_factor",
            title="dilution factor",
            scale=alt.Scale(nice=False, padding=5, type="log"),
        ),
        alt.Y(
            "frac_infectivity",
            title="fraction infectivity",
            scale=alt.Scale(nice=False, padding=5),
        ),
        alt.Column(
            "ceiling_applied",
            sort="descending",
            title=None,
            header=alt.Header(labelFontSize=13, labelFontStyle="bold", labelPadding=2),
        ),
        alt.Row(
            "serum_replicate",
            title=None,
            spacing=3,
            header=alt.Header(labelFontSize=13, labelFontStyle="bold"),
        ),
        alt.Detail("barcode"),
        alt.Shape(
            "fails_qc",
            title=f"fails {qc_thresholds['max_frac_infectivity_per_viral_barcode_well']=}",
            legend=alt.Legend(titleLimit=500, orient="bottom"),
        ),
        color=alt.condition(
            barcode_selection, alt.value("black"), alt.value("MediumBlue")
        ),
        strokeWidth=alt.condition(barcode_selection, alt.value(3), alt.value(1)),
        opacity=alt.condition(barcode_selection, alt.value(1), alt.value(0.25)),
        tooltip=[
            (
                alt.Tooltip(c, format=".3g")
                if frac_infectivity_chart_df[c].dtype == float
                else c
            )
            for c in frac_infectivity_chart_df.columns
        ],
    )
    .mark_line(point=True)
    .properties(
        height=150,
        width=250,
        title=f"Fraction infectivities for {plate}",
    )
    .interactive(bind_x=False)
    .configure_axis(grid=False)
    .configure_legend(titleLimit=1000)
    .configure_point(size=50)
    .resolve_scale(x="independent", y="independent")
)

display(frac_infectivity_chart)

# drop barcode / wells failing QC
max_frac_infectivity_per_viral_barcode_well_drops = list(
    frac_infectivity_chart_df.query("fails_qc")[["barcode", "well"]]
    .drop_duplicates()
    .itertuples(index=False, name=None)
)
print(
    f"\nDropping {len(max_frac_infectivity_per_viral_barcode_well_drops)} barcode-wells for failing "
    f"{qc_thresholds['max_frac_infectivity_per_viral_barcode_well']=}: "
    + str(max_frac_infectivity_per_viral_barcode_well_drops)
)
qc_drops["barcode_wells"].update(
    {
        w: "max_frac_infectivity_per_viral_barcode_well"
        for w in max_frac_infectivity_per_viral_barcode_well_drops
    }
)
frac_infectivity = frac_infectivity[
    ~frac_infectivity.assign(
        barcode_well=lambda x: x.apply(lambda r: (r["barcode"], r["well"]), axis=1)
    )["barcode_well"].isin(qc_drops["barcode_wells"])
]

Dropping 191 barcode-wells for failing qc_thresholds['max_frac_infectivity_per_viral_barcode_well']=4: [('ATGGATATGCTATCAG', 'D2'), ('ATTACCTAAGTGAAAT', 'D2'), ('ACGGCAAGTGATGAAA', 'D2'), ('ATAAGAGAGCATCTCT', 'D2'), ('AGTACTTCGTACTCCT', 'D2'), ('TTCTATAGTACTTCTA', 'D2'), ('GACAATATACCCCCAT', 'D2'), ('AAAGCCCTAGTTAAGT', 'D2'), ('AATTGTGAATGCCACA', 'D2'), ('TCCGAAAGACCAAAAC', 'D2'), ('CCACATAGGCGTTTTT', 'D3'), ('ACTAAAGGCATAGTAG', 'D4'), ('AAACTGATTAATGATG', 'D4'), ('ACGCCCCCACTTCATG', 'D4'), ('TAGGACTACAGAGAAC', 'C4'), ('AGAAAGCTGTTAATAC', 'C4'), ('GACAATATACCCCCAT', 'C4'), ('CCAAATTTTAACTGTG', 'C4'), ('CTGAACTTGTCGATAT', 'C4'), ('GAATATAAATGGGCAT', 'D5'), ('TGGGAAATCATTGGTA', 'C5'), ('TAGGACTACAGAGAAC', 'C5'), ('TTTTAGATAAACTTAG', 'C5'), ('GTATCCCCACGAAAGT', 'C5'), ('GACAATATACCCCCAT', 'C5'), ('AATAAAATGCATGGGT', 'C5'), ('GGCTTGAATGATATTG', 'C5'), ('TATGGCCTATAGGTTC', 'C5'), ('CTGAACTTGTCGATAT', 'C5'), ('GAATATAAATGGGCAT', 'C5'), ('GCATCCTTTTCCTGTT', 'F6'), ('GCCTTGCATTAGGAAC', 'E6'), ('GATAATAGAAAGTGTA', 'E6'), ('TTCTATGTTTCTTTAA', 'E6'), ('CGGCAGGATATTGCAG', 'E6'), ('AGTAAACATGCATTGG', 'E6'), ('TGCATTAATTCAACTA', 'E6'), ('CTCTCTACAGTATTGA', 'E6'), ('AATGAATGTATGCTAA', 'E6'), ('TCACAATTCATATAAT', 'E6'), ('CGCCTGTAAAAATTCG', 'E6'), ('GTCACTATATGATGTC', 'E6'), ('ACTTATGCTCTTGATA', 'E6'), ('TTATAAAAACAAATCG', 'E6'), ('TAGGACTACAGAGAAC', 'E6'), ('AGAACAATTATTGTTA', 'E6'), ('GTATCCCCACGAAAGT', 'E6'), ('ATATGTGCTAACAAAA', 'E6'), ('CACGAGTTGGTGGGTT', 'E6'), ('TATTCAACATTCTCTA', 'E6'), ('AACATGTGTAAGCGGT', 'E6'), ('AAATTAAAGAGGTTAA', 'E6'), ('TTTTAGATAAACTTAG', 'E6'), ('TGGGAAATCATTGGTA', 'E6'), ('TATGACATTAGAAACA', 'E6'), ('AATTTTAGGACGCCGT', 'E6'), ('ATTACCTAAGTGAAAT', 'E6'), ('AAGATGAATGTCTTAA', 'E6'), ('TAGTAATCTAAAGTAA', 'E6'), ('TGTGATAGACCTAGAA', 'E6'), ('GTTTCTGCCTTACAAA', 'E6'), ('TACTAAGTAAGAGCAA', 'E6'), ('AGACGCTTCGGCCCAA', 'E6'), ('AGCCCTTAAGACATTA', 'E6'), ('CTTCAAAGACTGATTA', 'E6'), ('ACTACCGCTAATATGC', 'E6'), ('ATACGTATTGATATCT', 'E6'), ('ATAGGCATGCTTACTG', 'E6'), ('AAACATATTGGCGTTT', 'E6'), ('AAACTGATTAATGATG', 'E6'), ('CTCCTAATAAAAAACT', 'E6'), ('ATAAGAGAGCATCTCT', 'E6'), ('AAAGCTCTTTTCGTTC', 'E6'), ('GTAATACTGATGGGCA', 'E6'), ('GTCCACAACAGTTGAA', 'E6'), ('GACAATATACCCCCAT', 'E6'), ('AATAAAATGCATGGGT', 'E6'), ('CTCATATTCCTATGAC', 'E6'), ('GAAAATGATCCACATT', 'E6'), ('GGCTTGAATGATATTG', 'E6'), ('AAATAATGAGTTTATA', 'E6'), ('GTCAAATAAATTTTCA', 'E6'), ('TGGCCTGCTTCGGAAA', 'E6'), ('TCATTGTCAATATTGA', 'E6'), ('ACCCCTTCAGAAGTTA', 'E6'), ('ATCTAATAATTATGGT', 'E6'), ('CCATAACTGTATATGG', 'E6'), ('AGTACTTCGTACTCCT', 'E6'), ('CCACATAGGCGTTTTT', 'E6'), ('CCAAGCTAGGTCGACA', 'E6'), ('GGATTGTAATAAATCA', 'E6'), ('AAAGCCCTAGTTAAGT', 'E6'), ('AATCCATTAGTTCAGC', 'E6'), ('GCATCCTTTTCCTGTT', 'E6'), ('TCCGAAAGACCAAAAC', 'E6'), ('AAAATCATTCGACTCT', 'E6'), ('ATTACCTAAGTGAAAT', 'D6'), ('ACGCCCCCACTTCATG', 'D6'), ('CCATAACTGTATATGG', 'D6'), ('ACGGCAAGTGATGAAA', 'C6'), ('CCAAATTTTAACTGTG', 'C6'), ('CCATAACTGTATATGG', 'C6'), ('CCACATAGGCGTTTTT', 'C6'), ('GCGGTATTCATTAATA', 'E7'), ('CAGAACACGATGGAAA', 'E7'), ('GGACATCTTTCAAGCA', 'E7'), ('ACTAATCGCAACTAGA', 'E7'), ('CATGCAATTCAGAGGG', 'E7'), ('TTCTATGTTTCTTTAA', 'E7'), ('TAACAGTTAGACAAAA', 'E7'), ('ATATCAAGACTAGATG', 'E7'), ('GTATCCCCACGAAAGT', 'E7'), ('CTGGAATGCAACGGGT', 'E7'), ('GTAGAAATCATAGGCG', 'E7'), ('TCACAATTCATATAAT', 'E7'), ('GGATTACACGATACCG', 'E7'), ('GTAAAGACCTTCGGAG', 'E7'), ('ATTATGGAAATAATGA', 'E7'), ('CTCTCTACAGTATTGA', 'E7'), ('CCTTGCGGTGTTTAAC', 'E7'), ('TGCCATCTGTGCATTA', 'E7'), ('CGCCTGTAAAAATTCG', 'E7'), ('CCCCAACCTATCGTAA', 'E7'), ('ACTTAGGAAGGTAAAC', 'E7'), ('TTATAAAAACAAATCG', 'E7'), ('ACTAAAGGCATAGTAG', 'E7'), ('CGCAGATATCATAGAA', 'E7'), ('TTACCTCTGAGAAACG', 'E7'), ('ATATGTGCTAACAAAA', 'E7'), ('GTCTAACAAGAATGTA', 'E7'), ('ACTTATGCTCTTGATA', 'E7'), ('AGAACAATTATTGTTA', 'E7'), ('CGCATGCAATTATAAA', 'E7'), ('TAGGACTACAGAGAAC', 'E7'), ('CACGAGTTGGTGGGTT', 'E7'), ('TTTTAGATAAACTTAG', 'E7'), ('TGAATGATTCTCCTTT', 'E7'), ('ATAGCATGCGATTTTA', 'E7'), ('CAAGGAAGTCAGGATT', 'E7'), ('CCTGTTTTTAGACGAA', 'E7'), ('TATTCAACATTCTCTA', 'E7'), ('AGAAAGCTGTTAATAC', 'E7'), ('GTCATATTCGATTACA', 'E7'), ('AAAATGCTGGGGTATA', 'E7'), ('AAGATGAATGTCTTAA', 'E7'), ('TAGTAATCTAAAGTAA', 'E7'), ('CTCCTAATAAAAAACT', 'E7'), ('TAAAGACAAAAAAACC', 'E7'), ('TTCTATAGTACTTCTA', 'E7'), ('TAACCGGGGAATCATT', 'E7'), ('GAGAGTACGGCACTGA', 'E7'), ('AGGAAAGAAACTGGAG', 'E7'), ('AAACTGATTAATGATG', 'E7'), ('GTCCACAACAGTTGAA', 'E7'), ('ACGGCAAGTGATGAAA', 'E7'), ('CAATCCGCTTGAATAC', 'E7'), ('TAACCGCTTCAATATA', 'E7'), ('CTTCAAAGACTGATTA', 'E7'), ('AATAAAATGCATGGGT', 'E7'), ('TGAAGCCATGAGTATC', 'E7'), ('GTAATACTGATGGGCA', 'E7'), ('CTCATATTCCTATGAC', 'E7'), ('TGTTTTACATTAGATG', 'E7'), ('CGATGCACTCGTAAGT', 'E7'), ('AAGATTACCAAATTAT', 'E7'), ('GAAAATGATCCACATT', 'E7'), ('TCTATCATCGCCGTTA', 'E7'), ('AGCATCACGTCAGTCT', 'E7'), ('CAGTCGCATTGAACCT', 'E7'), ('ATACGTATTGATATCT', 'E7'), ('CTGTAAAAAGCGTTAA', 'E7'), ('CCCTATCCCAGAACCT', 'E7'), ('TATGGCCTATAGGTTC', 'E7'), ('AAATAATGAGTTTATA', 'E7'), ('CAGATATGAGAGAGCA', 'E7'), ('CTGAACTTGTCGATAT', 'E7'), ('TGGCCTGCTTCGGAAA', 'E7'), ('ACGCCCCCACTTCATG', 'E7'), ('ATCCCATCAACAAAAT', 'E7'), ('CCATAACTGTATATGG', 'E7'), ('CCACATAGGCGTTTTT', 'E7'), ('GCATCCTTTTCCTGTT', 'E7'), ('AATTGTGAATGCCACA', 'E7'), ('AATCCATTAGTTCAGC', 'E7'), ('CCATAACTGTATATGG', 'D7'), ('AATCCGAAATTTATTC', 'D7'), ('GAATATAAATGGGCAT', 'C7'), ('CCACATAGGCGTTTTT', 'B7'), ('GAATATAAATGGGCAT', 'B7'), ('AATTGTGAATGCCACA', 'C8'), ('CCACATAGGCGTTTTT', 'D9')]

Check how many dilutions we have per barcode / serum-replicate:

n_dilutions = (
    frac_infectivity.groupby(["serum_replicate", "strain", "barcode"], as_index=False)
    .aggregate(**{"number of dilutions": pd.NamedAgg("dilution_factor", "nunique")})
    .assign(
        fails_qc=lambda x: (
            x["number of dilutions"]
            < qc_thresholds["min_dilutions_per_barcode_serum_replicate"]
        ),
    )
)

n_dilutions_chart = (
    alt.Chart(n_dilutions)
    .add_params(barcode_selection)
    .encode(
        alt.X("number of dilutions", scale=alt.Scale(nice=False, padding=4)),
        alt.Y("strain", title=None),
        alt.Column(
            "serum_replicate",
            title=None,
            header=alt.Header(labelFontSize=12, labelFontStyle="bold", labelPadding=0),
        ),
        alt.Fill(
            "fails_qc",
            title=f"fails {qc_thresholds['min_dilutions_per_barcode_serum_replicate']=}",
            legend=alt.Legend(titleLimit=500, orient="bottom"),
        ),
        strokeWidth=alt.condition(barcode_selection, alt.value(2), alt.value(0)),
        size=alt.condition(barcode_selection, alt.value(55), alt.value(35)),
        tooltip=[
            alt.Tooltip(c, format=".3g") if n_dilutions[c].dtype == float else c
            for c in n_dilutions.columns
        ],
    )
    .mark_circle(stroke="black", strokeOpacity=1, fillOpacity=0.45)
    .properties(
        height=alt.Step(10),
        width=120,
        title=alt.TitleParams(
            "number of dilutions for each barcode for each serum-replicate", dy=-2
        ),
    )
)

display(n_dilutions_chart)

# drop barcode / serum-replicates failing QC
min_dilutions_per_barcode_serum_replicate_drops = list(
    n_dilutions.query("fails_qc")[["barcode", "serum_replicate"]].itertuples(
        index=False, name=None
    )
)
print(
    f"\nDropping {len(min_dilutions_per_barcode_serum_replicate_drops)} barcode/serum-replicates for failing "
    f"{qc_thresholds['min_dilutions_per_barcode_serum_replicate']=}: "
    + str(min_dilutions_per_barcode_serum_replicate_drops)
)
qc_drops["barcode_serum_replicates"].update(
    {
        w: "min_dilutions_per_barcode_serum_replicate"
        for w in min_dilutions_per_barcode_serum_replicate_drops
    }
)
frac_infectivity = frac_infectivity[
    ~frac_infectivity.assign(
        barcode_serum_replicate=lambda x: x.apply(
            lambda r: (r["barcode"], r["serum_replicate"]), axis=1
        )
    )["barcode_serum_replicate"].isin(qc_drops["barcode_serum_replicates"])
]

Dropping 0 barcode/serum-replicates for failing qc_thresholds['min_dilutions_per_barcode_serum_replicate']=5: []

Fit neutralization curves without applying QC to curves

First fit curves to all serum replicates, then we will apply QC on the curve fits. Note that the fitting is done to the fraction infectivities with the ceiling:

fits_noqc = neutcurve.CurveFits(
    frac_infectivity.rename(
        columns={
            "frac_infectivity_ceiling": "fraction infectivity",
            "concentration": "serum concentration",
        }
    ),
    conc_col="serum concentration",
    fracinf_col="fraction infectivity",
    virus_col="strain",
    serum_col="serum_replicate",
    replicate_col="barcode",
    fixtop=curvefit_params["fixtop"],
    fixbottom=curvefit_params["fixbottom"],
    fixslope=curvefit_params["fixslope"],
)

Determine which fits fail the curve fitting QC, and plot them. Note the plot indicates as failing QC any barcode / serum-replicate that fails, even if we are also specified to ignore the QC for that one (so it will not be removed later):

goodness_of_fit = curvefit_qc["goodness_of_fit"]

fit_params_noqc = (
    frac_infectivity.groupby(["serum_replicate", "barcode"], as_index=False)
    .aggregate(max_frac_infectivity=pd.NamedAgg("frac_infectivity_ceiling", "max"))
    .merge(
        fits_noqc.fitParams(average_only=False, no_average=True)[
            ["serum", "virus", "replicate", "r2", "rmsd"]
        ].rename(columns={"serum": "serum_replicate", "replicate": "barcode"}),
        validate="one_to_one",
    )
    .assign(
        fails_max_frac_infectivity_at_least=lambda x: (
            x["max_frac_infectivity"] < curvefit_qc["max_frac_infectivity_at_least"]
        ),
        fails_goodness_of_fit=lambda x: (
            (x["r2"] < goodness_of_fit["min_R2"])
            & (x["rmsd"] > goodness_of_fit["max_RMSD"])
        ),
        fails_qc=lambda x: (
            x["fails_max_frac_infectivity_at_least"] | x["fails_goodness_of_fit"]
        ),
        ignore_qc=lambda x: x.apply(
            lambda r: (
                (
                    r["serum_replicate"]
                    in curvefit_qc["serum_replicates_ignore_curvefit_qc"]
                )
                or (
                    (r["barcode"], r["serum_replicate"])
                    in curvefit_qc["barcode_serum_replicates_ignore_curvefit_qc"]
                )
            ),
            axis=1,
        ),
    )
)

print(f"Plotting barcode / serum-replicates that fail {curvefit_qc=}\n")

for prop, col in [
    ("max frac infectivity", "max_frac_infectivity"),
    ("curve fit R2", "r2"),
    ("curve fit RMSD", "rmsd"),
]:
    fit_params_noqc_chart = (
        alt.Chart(fit_params_noqc)
        .add_params(barcode_selection)
        .encode(
            alt.X(col, title=prop, scale=alt.Scale(nice=False, padding=4)),
            alt.Y("virus", title=None),
            alt.Fill("fails_qc"),
            alt.Column(
                "serum_replicate",
                title=None,
                header=alt.Header(
                    labelFontSize=12, labelFontStyle="bold", labelPadding=0
                ),
            ),
            strokeWidth=alt.condition(barcode_selection, alt.value(2), alt.value(0)),
            size=alt.condition(barcode_selection, alt.value(55), alt.value(35)),
            tooltip=[
                alt.Tooltip(c, format=".3g") if fit_params_noqc[c].dtype == float else c
                for c in fit_params_noqc.columns
            ],
        )
        .mark_circle(stroke="black", strokeOpacity=1, fillOpacity=0.55)
        .properties(
            height=alt.Step(10),
            width=120,
            title=alt.TitleParams(f"{prop} for each barcode serum-replicate", dy=-2),
        )
    )
    display(fit_params_noqc_chart)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: invalid value encountered in power
  return b + (t - b) / (1 + (c / m) ** s)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: invalid value encountered in power
  return b + (t - b) / (1 + (c / m) ** s)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: divide by zero encountered in divide
  return b + (t - b) / (1 + (c / m) ** s)

Plotting barcode / serum-replicates that fail curvefit_qc={'max_frac_infectivity_at_least': 0, 'goodness_of_fit': {'min_R2': 0.7, 'max_RMSD': 0.2}, 'serum_replicates_ignore_curvefit_qc': [], 'barcode_serum_replicates_ignore_curvefit_qc': []}

Now get all barcode / serum-replicate pairs that fail any of the QC. Plot curves for just these virus / serum-replicates (we plot all barcodes for a virus even if just one fails QC), and then exclude any that are not specified to ignore the QC:

barcode_serum_replicates_fail_qc = fit_params_noqc.query("fails_qc").reset_index(
    drop=True
)
print(f"Here are barcode / serum-replicates that fail {curvefit_qc=}")
display(barcode_serum_replicates_fail_qc)

if len(barcode_serum_replicates_fail_qc):
    print("\nCurves for viruses and serum-replicates with at least one failed barcode:")
    fig, _ = fits_noqc.plotReplicates(
        sera=sorted(barcode_serum_replicates_fail_qc["serum_replicate"].unique()),
        viruses=sorted(barcode_serum_replicates_fail_qc["virus"].unique()),
        attempt_shared_legend=False,
        legendfontsize=8,
        titlesize=10,
        ticksize=10,
        ncol=6,
        draw_in_bounds=True,
    )
    display(fig)
    plt.close(fig)

# drop barcode / serum-replicates failing QC
for qc_filter in ["max_frac_infectivity_at_least", "goodness_of_fit"]:
    fits_qc_drops = list(
        fit_params_noqc.query(f"fails_{qc_filter} and (not ignore_qc)")[
            ["barcode", "serum_replicate"]
        ].itertuples(index=False, name=None)
    )
    print(
        f"\nDropping {len(fits_qc_drops)} barcode/serum-replicates for failing "
        f"{qc_filter}={curvefit_qc[qc_filter]}: " + str(fits_qc_drops)
    )
    qc_drops["barcode_serum_replicates"].update({w: qc_filter for w in fits_qc_drops})
    frac_infectivity = frac_infectivity[
        ~frac_infectivity.assign(
            barcode_serum_replicate=lambda x: x.apply(
                lambda r: (r["barcode"], r["serum_replicate"]), axis=1
            )
        )["barcode_serum_replicate"].isin(qc_drops["barcode_serum_replicates"])
    ]
    fit_params_noqc = fit_params_noqc[
        ~fit_params_noqc.assign(
            barcode_serum_replicate=lambda x: x.apply(
                lambda r: (r["barcode"], r["serum_replicate"]), axis=1
            )
        )["barcode_serum_replicate"].isin(qc_drops["barcode_serum_replicates"])
    ]

Here are barcode / serum-replicates that fail curvefit_qc={'max_frac_infectivity_at_least': 0, 'goodness_of_fit': {'min_R2': 0.7, 'max_RMSD': 0.2}, 'serum_replicates_ignore_curvefit_qc': [], 'barcode_serum_replicates_ignore_curvefit_qc': []}

	serum_replicate	barcode	max_frac_infectivity	virus	r2	rmsd	fails_max_frac_infectivity_at_least	fails_goodness_of_fit	fails_qc	ignore_qc
0	PENN23_y1987_s037_d0	AGCAGTAAATAAAATC	1.0	A/Oregon/Flu-OHSU-241140095/2023	0.133238	0.228065	False	True	True	False
1	PENN23_y1987_s037_d0	AGCCCGAAGAGCCCCT	1.0	A/California/75/2023	0.000000	0.203290	False	True	True	False
2	PENN23_y1996_s056_d0	AGCAGTAAATAAAATC	1.0	A/Oregon/Flu-OHSU-241140095/2023	0.640831	0.231250	False	True	True	False
3	PENN23_y1996_s056_d0	AGCCCGAAGAGCCCCT	1.0	A/California/75/2023	0.695338	0.246554	False	True	True	False

Curves for viruses and serum-replicates with at least one failed barcode:

Dropping 0 barcode/serum-replicates for failing max_frac_infectivity_at_least=0: []

Dropping 4 barcode/serum-replicates for failing goodness_of_fit={'min_R2': 0.7, 'max_RMSD': 0.2}: [('AGCAGTAAATAAAATC', 'PENN23_y1987_s037_d0'), ('AGCCCGAAGAGCCCCT', 'PENN23_y1987_s037_d0'), ('AGCAGTAAATAAAATC', 'PENN23_y1996_s056_d0'), ('AGCCCGAAGAGCCCCT', 'PENN23_y1996_s056_d0')]

Fit neutralization curves after applying QC

No we re-fit curves after applying all the QC:

fits_qc = neutcurve.CurveFits(
    frac_infectivity.rename(
        columns={
            "frac_infectivity_ceiling": "fraction infectivity",
            "concentration": "serum concentration",
        }
    ),
    conc_col="serum concentration",
    fracinf_col="fraction infectivity",
    virus_col="strain",
    serum_col="serum",
    replicate_col="plate_barcode",
    fixtop=curvefit_params["fixtop"],
    fixbottom=curvefit_params["fixbottom"],
    fixslope=curvefit_params["fixslope"],
)

fit_params_qc = fits_qc.fitParams(average_only=False, no_average=True)
assert len(fit_params_qc) <= len(
    fits_noqc.fitParams(average_only=False, no_average=True)
)

print(f"Assigning fits for this plate to {group}")
fit_params_qc.insert(0, "group", group)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: invalid value encountered in power
  return b + (t - b) / (1 + (c / m) ** s)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: invalid value encountered in power
  return b + (t - b) / (1 + (c / m) ** s)

/fh/fast/bloom_j/computational_notebooks/aloes/2025/flu_seqneut_pdmH1N1_2023-2024_VaccinatedCohorts/.snakemake/conda/de1e1a4710a5bd2a5adf38517b0654d5_/lib/python3.12/site-packages/neutcurve/hillcurve.py:1177: RuntimeWarning: divide by zero encountered in divide
  return b + (t - b) / (1 + (c / m) ** s)

Assigning fits for this plate to PennVaccineCohort

Plot all the curves that passed QC:

if fits_qc.sera:
    _ = fits_qc.plotReplicates(
        attempt_shared_legend=False,
        legendfontsize=8,
        titlesize=10,
        ticksize=10,
        ncol=6,
        draw_in_bounds=True,
    )
else:
    print("No sera passed QC.")

Save results to files

print(f"Writing fraction infectivities to {frac_infectivity_csv}")
(
    frac_infectivity[
        [
            "serum",
            "strain",
            "plate_barcode",
            "dilution_factor",
            "frac_infectivity_raw",
            "frac_infectivity_ceiling",
        ]
    ]
    .sort_values(["serum", "plate_barcode", "dilution_factor"])
    .to_csv(frac_infectivity_csv, index=False, float_format="%.4g")
)

print(f"\nWriting fit parameters to {fits_csv}")
(
    fit_params_qc.drop(columns=["nreplicates", "ic50_str"]).to_csv(
        fits_csv, index=False, float_format="%.4g"
    )
)

print(f"\nPickling neutcurve.CurveFits object for these data to {fits_pickle}")
with open(fits_pickle, "wb") as f:
    pickle.dump(fits_qc, f)

print(f"\nWriting QC drops to {qc_drops_yaml}")


def tup_to_str(x):
    return " ".join(x) if isinstance(x, tuple) else x


qc_drops_for_yaml = {
    key: {tup_to_str(key2): val2 for key2, val2 in val.items()}
    for key, val in qc_drops.items()
}
with open(qc_drops_yaml, "w") as f:
    yaml.YAML(typ="rt").dump(qc_drops_for_yaml, f)
print("\nHere are the QC drops:\n***************************")
yaml.YAML(typ="rt").dump(qc_drops_for_yaml, sys.stdout)

Writing fraction infectivities to results/plates/plate6/frac_infectivity.csv

Writing fit parameters to results/plates/plate6/curvefits.csv

Pickling neutcurve.CurveFits object for these data to results/plates/plate6/curvefits.pickle

Writing QC drops to results/plates/plate6/qc_drops.yml

Here are the QC drops:
***************************
wells:
  A12: manual_drop
  B12: manual_drop
  C12: manual_drop
  D12: manual_drop
  E12: manual_drop
  F12: manual_drop
  G12: manual_drop
  H12: manual_drop
barcodes:
  CCAGGAACAATATATC: min_neut_standard_frac_per_well
  CCCACCCCTGCCTCCC: min_neut_standard_frac_per_well
  GAGCAGTATCTGGTTA: min_neut_standard_frac_per_well
  TTGAAAGCCACAACAC: min_neut_standard_frac_per_well
barcode_wells:
  AAAATCATTCGACTCT G1: min_no_serum_count_per_viral_barcode_well
  ATGGATATGCTATCAG D2: max_frac_infectivity_per_viral_barcode_well
  ATTACCTAAGTGAAAT D2: max_frac_infectivity_per_viral_barcode_well
  ACGGCAAGTGATGAAA D2: max_frac_infectivity_per_viral_barcode_well
  ATAAGAGAGCATCTCT D2: max_frac_infectivity_per_viral_barcode_well
  AGTACTTCGTACTCCT D2: max_frac_infectivity_per_viral_barcode_well
  TTCTATAGTACTTCTA D2: max_frac_infectivity_per_viral_barcode_well
  GACAATATACCCCCAT D2: max_frac_infectivity_per_viral_barcode_well
  AAAGCCCTAGTTAAGT D2: max_frac_infectivity_per_viral_barcode_well
  AATTGTGAATGCCACA D2: max_frac_infectivity_per_viral_barcode_well
  TCCGAAAGACCAAAAC D2: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT D3: max_frac_infectivity_per_viral_barcode_well
  ACTAAAGGCATAGTAG D4: max_frac_infectivity_per_viral_barcode_well
  AAACTGATTAATGATG D4: max_frac_infectivity_per_viral_barcode_well
  ACGCCCCCACTTCATG D4: max_frac_infectivity_per_viral_barcode_well
  TAGGACTACAGAGAAC C4: max_frac_infectivity_per_viral_barcode_well
  AGAAAGCTGTTAATAC C4: max_frac_infectivity_per_viral_barcode_well
  GACAATATACCCCCAT C4: max_frac_infectivity_per_viral_barcode_well
  CCAAATTTTAACTGTG C4: max_frac_infectivity_per_viral_barcode_well
  CTGAACTTGTCGATAT C4: max_frac_infectivity_per_viral_barcode_well
  GAATATAAATGGGCAT D5: max_frac_infectivity_per_viral_barcode_well
  TGGGAAATCATTGGTA C5: max_frac_infectivity_per_viral_barcode_well
  TAGGACTACAGAGAAC C5: max_frac_infectivity_per_viral_barcode_well
  TTTTAGATAAACTTAG C5: max_frac_infectivity_per_viral_barcode_well
  GTATCCCCACGAAAGT C5: max_frac_infectivity_per_viral_barcode_well
  GACAATATACCCCCAT C5: max_frac_infectivity_per_viral_barcode_well
  AATAAAATGCATGGGT C5: max_frac_infectivity_per_viral_barcode_well
  GGCTTGAATGATATTG C5: max_frac_infectivity_per_viral_barcode_well
  TATGGCCTATAGGTTC C5: max_frac_infectivity_per_viral_barcode_well
  CTGAACTTGTCGATAT C5: max_frac_infectivity_per_viral_barcode_well
  GAATATAAATGGGCAT C5: max_frac_infectivity_per_viral_barcode_well
  GCATCCTTTTCCTGTT F6: max_frac_infectivity_per_viral_barcode_well
  GCCTTGCATTAGGAAC E6: max_frac_infectivity_per_viral_barcode_well
  GATAATAGAAAGTGTA E6: max_frac_infectivity_per_viral_barcode_well
  TTCTATGTTTCTTTAA E6: max_frac_infectivity_per_viral_barcode_well
  CGGCAGGATATTGCAG E6: max_frac_infectivity_per_viral_barcode_well
  AGTAAACATGCATTGG E6: max_frac_infectivity_per_viral_barcode_well
  TGCATTAATTCAACTA E6: max_frac_infectivity_per_viral_barcode_well
  CTCTCTACAGTATTGA E6: max_frac_infectivity_per_viral_barcode_well
  AATGAATGTATGCTAA E6: max_frac_infectivity_per_viral_barcode_well
  TCACAATTCATATAAT E6: max_frac_infectivity_per_viral_barcode_well
  CGCCTGTAAAAATTCG E6: max_frac_infectivity_per_viral_barcode_well
  GTCACTATATGATGTC E6: max_frac_infectivity_per_viral_barcode_well
  ACTTATGCTCTTGATA E6: max_frac_infectivity_per_viral_barcode_well
  TTATAAAAACAAATCG E6: max_frac_infectivity_per_viral_barcode_well
  TAGGACTACAGAGAAC E6: max_frac_infectivity_per_viral_barcode_well
  AGAACAATTATTGTTA E6: max_frac_infectivity_per_viral_barcode_well
  GTATCCCCACGAAAGT E6: max_frac_infectivity_per_viral_barcode_well
  ATATGTGCTAACAAAA E6: max_frac_infectivity_per_viral_barcode_well
  CACGAGTTGGTGGGTT E6: max_frac_infectivity_per_viral_barcode_well
  TATTCAACATTCTCTA E6: max_frac_infectivity_per_viral_barcode_well
  AACATGTGTAAGCGGT E6: max_frac_infectivity_per_viral_barcode_well
  AAATTAAAGAGGTTAA E6: max_frac_infectivity_per_viral_barcode_well
  TTTTAGATAAACTTAG E6: max_frac_infectivity_per_viral_barcode_well
  TGGGAAATCATTGGTA E6: max_frac_infectivity_per_viral_barcode_well
  TATGACATTAGAAACA E6: max_frac_infectivity_per_viral_barcode_well
  AATTTTAGGACGCCGT E6: max_frac_infectivity_per_viral_barcode_well
  ATTACCTAAGTGAAAT E6: max_frac_infectivity_per_viral_barcode_well
  AAGATGAATGTCTTAA E6: max_frac_infectivity_per_viral_barcode_well
  TAGTAATCTAAAGTAA E6: max_frac_infectivity_per_viral_barcode_well
  TGTGATAGACCTAGAA E6: max_frac_infectivity_per_viral_barcode_well
  GTTTCTGCCTTACAAA E6: max_frac_infectivity_per_viral_barcode_well
  TACTAAGTAAGAGCAA E6: max_frac_infectivity_per_viral_barcode_well
  AGACGCTTCGGCCCAA E6: max_frac_infectivity_per_viral_barcode_well
  AGCCCTTAAGACATTA E6: max_frac_infectivity_per_viral_barcode_well
  CTTCAAAGACTGATTA E6: max_frac_infectivity_per_viral_barcode_well
  ACTACCGCTAATATGC E6: max_frac_infectivity_per_viral_barcode_well
  ATACGTATTGATATCT E6: max_frac_infectivity_per_viral_barcode_well
  ATAGGCATGCTTACTG E6: max_frac_infectivity_per_viral_barcode_well
  AAACATATTGGCGTTT E6: max_frac_infectivity_per_viral_barcode_well
  AAACTGATTAATGATG E6: max_frac_infectivity_per_viral_barcode_well
  CTCCTAATAAAAAACT E6: max_frac_infectivity_per_viral_barcode_well
  ATAAGAGAGCATCTCT E6: max_frac_infectivity_per_viral_barcode_well
  AAAGCTCTTTTCGTTC E6: max_frac_infectivity_per_viral_barcode_well
  GTAATACTGATGGGCA E6: max_frac_infectivity_per_viral_barcode_well
  GTCCACAACAGTTGAA E6: max_frac_infectivity_per_viral_barcode_well
  GACAATATACCCCCAT E6: max_frac_infectivity_per_viral_barcode_well
  AATAAAATGCATGGGT E6: max_frac_infectivity_per_viral_barcode_well
  CTCATATTCCTATGAC E6: max_frac_infectivity_per_viral_barcode_well
  GAAAATGATCCACATT E6: max_frac_infectivity_per_viral_barcode_well
  GGCTTGAATGATATTG E6: max_frac_infectivity_per_viral_barcode_well
  AAATAATGAGTTTATA E6: max_frac_infectivity_per_viral_barcode_well
  GTCAAATAAATTTTCA E6: max_frac_infectivity_per_viral_barcode_well
  TGGCCTGCTTCGGAAA E6: max_frac_infectivity_per_viral_barcode_well
  TCATTGTCAATATTGA E6: max_frac_infectivity_per_viral_barcode_well
  ACCCCTTCAGAAGTTA E6: max_frac_infectivity_per_viral_barcode_well
  ATCTAATAATTATGGT E6: max_frac_infectivity_per_viral_barcode_well
  CCATAACTGTATATGG E6: max_frac_infectivity_per_viral_barcode_well
  AGTACTTCGTACTCCT E6: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT E6: max_frac_infectivity_per_viral_barcode_well
  CCAAGCTAGGTCGACA E6: max_frac_infectivity_per_viral_barcode_well
  GGATTGTAATAAATCA E6: max_frac_infectivity_per_viral_barcode_well
  AAAGCCCTAGTTAAGT E6: max_frac_infectivity_per_viral_barcode_well
  AATCCATTAGTTCAGC E6: max_frac_infectivity_per_viral_barcode_well
  GCATCCTTTTCCTGTT E6: max_frac_infectivity_per_viral_barcode_well
  TCCGAAAGACCAAAAC E6: max_frac_infectivity_per_viral_barcode_well
  AAAATCATTCGACTCT E6: max_frac_infectivity_per_viral_barcode_well
  ATTACCTAAGTGAAAT D6: max_frac_infectivity_per_viral_barcode_well
  ACGCCCCCACTTCATG D6: max_frac_infectivity_per_viral_barcode_well
  CCATAACTGTATATGG D6: max_frac_infectivity_per_viral_barcode_well
  ACGGCAAGTGATGAAA C6: max_frac_infectivity_per_viral_barcode_well
  CCAAATTTTAACTGTG C6: max_frac_infectivity_per_viral_barcode_well
  CCATAACTGTATATGG C6: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT C6: max_frac_infectivity_per_viral_barcode_well
  GCGGTATTCATTAATA E7: max_frac_infectivity_per_viral_barcode_well
  CAGAACACGATGGAAA E7: max_frac_infectivity_per_viral_barcode_well
  GGACATCTTTCAAGCA E7: max_frac_infectivity_per_viral_barcode_well
  ACTAATCGCAACTAGA E7: max_frac_infectivity_per_viral_barcode_well
  CATGCAATTCAGAGGG E7: max_frac_infectivity_per_viral_barcode_well
  TTCTATGTTTCTTTAA E7: max_frac_infectivity_per_viral_barcode_well
  TAACAGTTAGACAAAA E7: max_frac_infectivity_per_viral_barcode_well
  ATATCAAGACTAGATG E7: max_frac_infectivity_per_viral_barcode_well
  GTATCCCCACGAAAGT E7: max_frac_infectivity_per_viral_barcode_well
  CTGGAATGCAACGGGT E7: max_frac_infectivity_per_viral_barcode_well
  GTAGAAATCATAGGCG E7: max_frac_infectivity_per_viral_barcode_well
  TCACAATTCATATAAT E7: max_frac_infectivity_per_viral_barcode_well
  GGATTACACGATACCG E7: max_frac_infectivity_per_viral_barcode_well
  GTAAAGACCTTCGGAG E7: max_frac_infectivity_per_viral_barcode_well
  ATTATGGAAATAATGA E7: max_frac_infectivity_per_viral_barcode_well
  CTCTCTACAGTATTGA E7: max_frac_infectivity_per_viral_barcode_well
  CCTTGCGGTGTTTAAC E7: max_frac_infectivity_per_viral_barcode_well
  TGCCATCTGTGCATTA E7: max_frac_infectivity_per_viral_barcode_well
  CGCCTGTAAAAATTCG E7: max_frac_infectivity_per_viral_barcode_well
  CCCCAACCTATCGTAA E7: max_frac_infectivity_per_viral_barcode_well
  ACTTAGGAAGGTAAAC E7: max_frac_infectivity_per_viral_barcode_well
  TTATAAAAACAAATCG E7: max_frac_infectivity_per_viral_barcode_well
  ACTAAAGGCATAGTAG E7: max_frac_infectivity_per_viral_barcode_well
  CGCAGATATCATAGAA E7: max_frac_infectivity_per_viral_barcode_well
  TTACCTCTGAGAAACG E7: max_frac_infectivity_per_viral_barcode_well
  ATATGTGCTAACAAAA E7: max_frac_infectivity_per_viral_barcode_well
  GTCTAACAAGAATGTA E7: max_frac_infectivity_per_viral_barcode_well
  ACTTATGCTCTTGATA E7: max_frac_infectivity_per_viral_barcode_well
  AGAACAATTATTGTTA E7: max_frac_infectivity_per_viral_barcode_well
  CGCATGCAATTATAAA E7: max_frac_infectivity_per_viral_barcode_well
  TAGGACTACAGAGAAC E7: max_frac_infectivity_per_viral_barcode_well
  CACGAGTTGGTGGGTT E7: max_frac_infectivity_per_viral_barcode_well
  TTTTAGATAAACTTAG E7: max_frac_infectivity_per_viral_barcode_well
  TGAATGATTCTCCTTT E7: max_frac_infectivity_per_viral_barcode_well
  ATAGCATGCGATTTTA E7: max_frac_infectivity_per_viral_barcode_well
  CAAGGAAGTCAGGATT E7: max_frac_infectivity_per_viral_barcode_well
  CCTGTTTTTAGACGAA E7: max_frac_infectivity_per_viral_barcode_well
  TATTCAACATTCTCTA E7: max_frac_infectivity_per_viral_barcode_well
  AGAAAGCTGTTAATAC E7: max_frac_infectivity_per_viral_barcode_well
  GTCATATTCGATTACA E7: max_frac_infectivity_per_viral_barcode_well
  AAAATGCTGGGGTATA E7: max_frac_infectivity_per_viral_barcode_well
  AAGATGAATGTCTTAA E7: max_frac_infectivity_per_viral_barcode_well
  TAGTAATCTAAAGTAA E7: max_frac_infectivity_per_viral_barcode_well
  CTCCTAATAAAAAACT E7: max_frac_infectivity_per_viral_barcode_well
  TAAAGACAAAAAAACC E7: max_frac_infectivity_per_viral_barcode_well
  TTCTATAGTACTTCTA E7: max_frac_infectivity_per_viral_barcode_well
  TAACCGGGGAATCATT E7: max_frac_infectivity_per_viral_barcode_well
  GAGAGTACGGCACTGA E7: max_frac_infectivity_per_viral_barcode_well
  AGGAAAGAAACTGGAG E7: max_frac_infectivity_per_viral_barcode_well
  AAACTGATTAATGATG E7: max_frac_infectivity_per_viral_barcode_well
  GTCCACAACAGTTGAA E7: max_frac_infectivity_per_viral_barcode_well
  ACGGCAAGTGATGAAA E7: max_frac_infectivity_per_viral_barcode_well
  CAATCCGCTTGAATAC E7: max_frac_infectivity_per_viral_barcode_well
  TAACCGCTTCAATATA E7: max_frac_infectivity_per_viral_barcode_well
  CTTCAAAGACTGATTA E7: max_frac_infectivity_per_viral_barcode_well
  AATAAAATGCATGGGT E7: max_frac_infectivity_per_viral_barcode_well
  TGAAGCCATGAGTATC E7: max_frac_infectivity_per_viral_barcode_well
  GTAATACTGATGGGCA E7: max_frac_infectivity_per_viral_barcode_well
  CTCATATTCCTATGAC E7: max_frac_infectivity_per_viral_barcode_well
  TGTTTTACATTAGATG E7: max_frac_infectivity_per_viral_barcode_well
  CGATGCACTCGTAAGT E7: max_frac_infectivity_per_viral_barcode_well
  AAGATTACCAAATTAT E7: max_frac_infectivity_per_viral_barcode_well
  GAAAATGATCCACATT E7: max_frac_infectivity_per_viral_barcode_well
  TCTATCATCGCCGTTA E7: max_frac_infectivity_per_viral_barcode_well
  AGCATCACGTCAGTCT E7: max_frac_infectivity_per_viral_barcode_well
  CAGTCGCATTGAACCT E7: max_frac_infectivity_per_viral_barcode_well
  ATACGTATTGATATCT E7: max_frac_infectivity_per_viral_barcode_well
  CTGTAAAAAGCGTTAA E7: max_frac_infectivity_per_viral_barcode_well
  CCCTATCCCAGAACCT E7: max_frac_infectivity_per_viral_barcode_well
  TATGGCCTATAGGTTC E7: max_frac_infectivity_per_viral_barcode_well
  AAATAATGAGTTTATA E7: max_frac_infectivity_per_viral_barcode_well
  CAGATATGAGAGAGCA E7: max_frac_infectivity_per_viral_barcode_well
  CTGAACTTGTCGATAT E7: max_frac_infectivity_per_viral_barcode_well
  TGGCCTGCTTCGGAAA E7: max_frac_infectivity_per_viral_barcode_well
  ACGCCCCCACTTCATG E7: max_frac_infectivity_per_viral_barcode_well
  ATCCCATCAACAAAAT E7: max_frac_infectivity_per_viral_barcode_well
  CCATAACTGTATATGG E7: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT E7: max_frac_infectivity_per_viral_barcode_well
  GCATCCTTTTCCTGTT E7: max_frac_infectivity_per_viral_barcode_well
  AATTGTGAATGCCACA E7: max_frac_infectivity_per_viral_barcode_well
  AATCCATTAGTTCAGC E7: max_frac_infectivity_per_viral_barcode_well
  CCATAACTGTATATGG D7: max_frac_infectivity_per_viral_barcode_well
  AATCCGAAATTTATTC D7: max_frac_infectivity_per_viral_barcode_well
  GAATATAAATGGGCAT C7: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT B7: max_frac_infectivity_per_viral_barcode_well
  GAATATAAATGGGCAT B7: max_frac_infectivity_per_viral_barcode_well
  AATTGTGAATGCCACA C8: max_frac_infectivity_per_viral_barcode_well
  CCACATAGGCGTTTTT D9: max_frac_infectivity_per_viral_barcode_well
barcode_serum_replicates:
  AGCAGTAAATAAAATC PENN23_y1987_s037_d0: goodness_of_fit
  AGCCCGAAGAGCCCCT PENN23_y1987_s037_d0: goodness_of_fit
  AGCAGTAAATAAAATC PENN23_y1996_s056_d0: goodness_of_fit
  AGCCCGAAGAGCCCCT PENN23_y1996_s056_d0: goodness_of_fit
serum_replicates:
  PENN23_y1964_s038_d0: manual_drop
  PENN23_y1964_s038_d28: manual_drop